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SOP (Standard Operating Procedure) for FTIR 7th Semester B.Pharmacy ,BP701T Instrumental Methods of Analysis,BPharmacy,Pharmaceutical Engineering,Standard Operating Procedure (SOP),Practical and Experiments,

SOP (Standard Operating Procedure) for FTIR

B.Pharmacy, 7th Semester, 2022 (2021-2022) -

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218 2 Report Jan 18, 2022

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SOP (Standard Operating Procedure) for FTIR



SWAMI DAYANAND POST GRADUATE INSTITUTE OF
PHARMACEUTICAL SCIENCES ROHTAK
SOP for FTIR (Fourier Transform Infrared Spectroscopy)
PROCEDURE FOR OPERATION AND CALIBRATION OF FTIR:
Start-up of FTIR Software
● Clean the Instrument and surroundings.
● Connect the instrument with the power supply.
● Switch on the instrument and Computer system.
● Double click on “IR Solution” Icon.
● After No. of internal operation.
● Select the “Measure” and select Measurement from the main menu, further select “initialize”
from the measurement tab.
● Press “Yes” then following screen will appear:
● Select File to load standard method for analysis or fill the desired parameter of the method.
● Click on file,
● Select the above option to enter the main window and select the standard method for analysis
or prepare new method as per STP.
● After selecting the standard method, double click on method or Open it from main window.
A dialog box will appear and ask about downloading parameter of Method.
● Press “OK” to download the method parameters
● After completion of method downloading,
● Click on “BKG” for background correction. A dialog box will appear.
● Press “OK” if the Sample compartment is ready for Background.
● Select measure from the above screen window, fill all the required (sample/standards
information) in comment/Date file and select sample for sample analysis.
● For the Sample Analysis click on the Data path file and give the appropriate file path for
saving the data.
● Copy the file name and paste the data in the comment and click on the sample tab.
● After scanning the sample click on “Calculate” and then “OK” on the Peak Table.
● The number of peaks in the Peak Table must be between 15 to 25.
● To adjust the number of peaks, click on “Calculate” and then increase or decrease the “Min
Area” and then click on “OK”.
● Click on “Window” from the main menu bar and select “Join Visible”.
● Then click on “Manipulation 2” and select Purity.
Downloaded from HK Technical PGIMS (pgims.hktechnical.com)
● Right click on the sample and select send to source similarly right click on the reference and
select send to reference.
● Send to reference
● Click on “Calc”, then “OK”, select the print command from the main window.
● Click on “OK” and select the desired report template for the Peak purity Graph as shown
below.
● Select the Report format and click on “Open” or double click on Report format. Press ‘OK’
for printing the document.
● For Peak table: select the Manipulation 1 and select Peak table.
o After selecting the peak table,
o Click on “Calc” then “OK”.
● Select “Print” from the above screen.
● Select the report format as ‘peak table’ and print. As per the above-described procedure.


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