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Plant Tissue Culture 3rd Semester B.Pharmacy Lecture Notes,BP303T Pharmaceutical Microbiology,Handwritten Notes,B. Pharmacy 3rd sem,BP303T Pharmaceutical Microbiology,Plant tissue Culture,

Plant Tissue Culture

B.Pharmacy, 3rd Semester, 2023 (2022-2023) - Lecture Notes

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Plant Tissue Culture

Plant Tissue Culture (PTC)

Definition

Importance

Principle

General Procedure

Advantages

History

Nutritional Requirement

  • Inorganic Nutrients
    • Micronutrients
      • Iron
      • Copper
      • Zinc
      • Cobalt
      • Manganese
      • Molybdenum
      • Chlorine
    • Macronutrients
      • Nitrogen
      • Potassium
      • Calcium
      • Magnesium
      • Phosphorous
      • Sulfur
  • Carbon Energy Source
  • Growth Regulators
    • Auxins
    • Cytokinins
    • Gibberllins
    • Abscisic
  • Organic Supplements
    • Vitamins
    • Amino Acids
    • Antibiotics
  • Solidifying Agents
  • pH of medium

General Procedure Involved in Plant Tissue Culture and their Maintanance

  • Sterilisation of Glasswares
  • Preparation and Sterilisation of Explant
  • Production of Callus from Explant
  • Proliferation of Callus
  • Subcutting of Callus
  • Suspension Culture

Types of Culture

  • Callus Culture
    • Lag Phase
    • Log/Exponential Phase
    • Decline Phase
    • Stationary Phase
  • Organ Culture
    • Embryo Culture: Procedure, Advantage, Application
    • Root Culture
    • Seed Culture
    • Nucleus Culture
      • Flower Culture: Procedure
      • Anther and Pollen Culture: Procedure
  • Protoplast Culture: Procedure
  • Cell Culture
    • Single Cell Culture
    • Suspension Culture
    • Batch Culture
      • Cycle of Batch Culture: Lag Phase, Log Phase, Deceleration Phase, Linear Phase, Stationary Phase.
    • Continous Culture

Application of Plant Tissue Culture in Pharmacognosy

  1. Production of microorganism free plants.
  2. Production of secondary metabolites.
  3. Study of plant biology.
  4. Germplasm Storage
  5. Production of haploid plants
  6. Selection of mutant cells
  7. Micropropagation
  8. Immobilization of plant cells

Edible Vaccine

  • Definition
  • Plants Used for Edible Vaccine
  • Advantages
  • Limitation

 

 

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