M.Pharm-II Semester sessional exam (2022) assesses advanced knowledge in Proteins and Protein Formulation, covering:
Protein Isolation & Purification: Techniques like chromatography (HPLC, affinity) and centrifugation.
Peptide Stability: Factors affecting degradation (pH, temperature) and stabilization strategies (lyophilization, excipients).
Protein Engineering:
Gene Shuffling: Creating genetic diversity for improved protein function.
Directed Evolution: Iterative mutagenesis for optimizing enzymatic activity or stability.
Formulation Relevance: Links to biopharmaceutical development (e.g., monoclonal antibodies, insulin analogs).
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