Flame Photometry & Atomic absorption spectroscopy notes B.Pharmacy 7th Semester 2024 (2023-2024) Previous Year's Question Papers/Notes Download - HK Technical PGIMS

Flame Photometry & Atomic Absorption Spectroscopy  

Flame Photometry

Principle

Instrumentations

• Burners
  • Mecker Burner
  • Total Consumption Burner
  • Premixed Burner
• Nebulizer
• Monochromators
  • Prism 
  • Gratings
• Detectors
  • PMT
• Read out Devices

Interferences

• Spectral Interferences
• vaporization Interferences
• Ionisation Interferences

Applications

• In identification of alkali and alkali earthen metals.
• Detect the presence of various metals in fruit juices, soft drinks.
• Analysis of biological fluids and tissues.
• Cement Analysis.
• Used in the study of equillibrium constant, ion exchange resin etc...
• Analysis of geological materials such as clay and minerals...

Atomic Absorption Spectroscopy (AAS)

Principle

Interferences

• Physical
  • Background Absorption
  • Spectral line Interference
• Anionic
• Vaporization
• Ionisation

AAS Instrumentation

• Radiation Source
  • Hollow Cathode Lamp
  • Electrodeless Discharge Lamp
• Chopper
• Atomizers
• Nebulizers
• Monochromators
  • Prism
  • Gratings
• Detectors
  • PMT
• Amplifiers
• Recorder

Application of AAS

• In Quantitative Ananlysis.
• In Qualitative Analysis.
• Determination of metallic elements in food industry.
• Determination of metallic elements in biological materials.
• Determination cobalt in steel.
• In analysis of cement.
• Analysis of iron ores.
• Determination of calcium in saliva.
• Determination of calcium, magnesium and potassium in blood stream.
• Determination of lead in petrol etc...