Biotechenology: immunity mechanisms, vaccine production, and hybridoma technology B.Pharmacy 6th Semester 2023 (2022-2023) Previous Year's Question Papers/Notes Download - HK Technical PGIMS

B pharmacy 6th sem biotechnology 3 unit notes 

This document delves into immunity mechanisms, vaccine production, and hybridoma technology, covering both foundational and advanced concepts in immunology and biotech applications. Key highlights include:

• Types of Immunity:

  • Active vs. Passive Immunity: Differences in antibody production (self-generated vs. transferred).

  • Humoral & Cell-Mediated Immunity: Roles of B lymphocytes (antibodies) and T lymphocytes (cytotoxic/helper cells).

  • Hypersensitivity Reactions: Type I-IV classifications (e.g., allergies, cytotoxic responses).

• Vaccine Production:

  • Methods: Cultivation in fertile eggs, tissue cultures, and free-living animals.

  • Stability & Storage: Cold chain requirements (e.g., –58°F for varicella) and ICH stability testing.

  • Toxoid Preparation: Detoxification of bacterial toxins (e.g., diphtheria toxoid).

• Hybridoma Technology:

  • Monoclonal Antibody (mAb) Production: Steps from immunization to purification (in vitro/in vivo).

  • Applications:

    • Diagnostics: Pregnancy tests, cancer detection.

    • Therapeutics: Targeted cancer treatment, immunotoxins, autoimmune disease management.